产品与服务 - MACS细胞分离 - MACS Technology - 基本原理

MACS® MicroBeads——被广泛验证的可信赖的磁珠

  • 既可以分选高频细胞,又能高效分选稀有的低频细胞类型
  • 高效、灵活,可以进行大量细胞的分选
  • 可靠,无论基础科研领域还是临床应用领域,MACS磁珠均得到了广泛验证
  • 至今已有超过35,000 例临床细胞治疗应用用到了MACS磁珠

MACS磁珠是非常小的磁珠,直径只有50nm,分选过程中,磁珠的结合对细胞的活化状态不会有影响,也不会占据太多细胞表位,对细胞大小也没有影响,因此,分选到的细胞可以直接用于下游实验。另外,MACS磁珠无毒、可以生物降解。因此,与大磁珠相比,分选后的细胞无需进行磁珠去除步骤,可以直接用于下游实验,如细胞培养、流式检测等。

目前只有MACS磁珠是胶体溶液,不会沉降,因此,细胞标记速度快、步骤少、效率高。

MACS磁珠的这些特征可以保留细胞表面分子的原始状态,让您的研究更客观、更准确!

了解更多MicroBeads 产品列表 。 

MACS MicroBeads are 50-nm superparamagnetic particles that are conjugated to highly specific antibodies against a particular antigen on the cell surface. Due to the small size, they do not activate cells and they will not saturate cell surface epitopes. Unlike larger beads, MACS MicroBeads do not have to be removed for any downstream application. MicroBeads are non-toxic and biodegradable. They have been tested according to the International Standard (ISO 10993) and USP guidelines for biocompatibility.
Only MACS MicroBeads are always in suspension (colloidal) – allowing fast binding kinetics and short labeling procedures. Unlike non-colloidal solutions or very large beads, you will never have to deal with clumping artifacts and you can count on superb quality and lot-to-lot consistency.

MACS Columns contain a matrix composed of ferromagnetic spheres covered with a cell-friendly coating. When placed on a magnetic separator, the spheres amplify the magnetic field by 10,000-fold, thus inducing a high gradient within the column. This is crucial for isolation of cells that are only minimally labeled, leaving enough epitopes free for concurrent antibody staining.
The space between the spheres is several times larger than primary and most cultured cells. This allows the cells to freely flow through the column. Magnetically labeled cells are held in suspension within the column and do not actually “bind” the column matrix. This suspension minimizes stress on the cells and allows for efficient sterile washing by avoiding cell aggregation.

MACS Separators are available in different formats to accommodate different column options.

The combination of MACS MicroBeads, columns, and the magnetic separator provide the perfect tool set for unmatched cell separation results.

Just three easy steps

Just three easy steps
It takes only a few simple steps to get pure cell populations Cells in a single-cell suspension are magnetically labeled with MACS MicroBeads. The sample is applied to a MACS Column placed in a MACS Separator.

The unlabeled cells pass through while the magnetically labeled cells are retained within the column. The flow-through can be collected as the unlabeled cell fraction. After a short washing step, the column is removed from the separator, and the magnetically labeled cells are eluted from the column. Thus, with MACS MicroBead Technology both labeled and unlabeled cells can easily be isolated with high purity and recovery.

Hands-on MACS Technology

Direct magnetic labeling

Direct labeling with MACS® MicroBeads is the fastest way of magnetic labeling. MACS MicroBeads specifically bind to antigens on the cell surface. Only one incubation step is required. Direct magnetic labeling requires a minimal number of washing steps and therefore minimizes cell loss.

Highly specific cell separation reagents for direct labeling of numerous cell types with MACS MicroBeads are available for human, mouse, rat, and non-human primate cells.

Indirect magnetic labeling

Indirect magnetic labeling is based on a two-step procedure. In a first step, the cells are labeled with a primary antibody directed against a cell surface marker.
In a second step, the cells are magnetically labeled with MACS MicroBeads, which either bind to the primary antibody or to a molecule that is conjugated to the primary antibody.
The primary antibody can either be unconjugated, biotinylated, or fluorochrome-conjugated. Accordingly, magnetic labeling is achieved with Anti-Immunoglobulin MicroBeads, Anti-Biotin MicroBeads, or Anti-Fluorochrome MicroBeads.



Indirect labeling can also be performed by using a cocktail of primary antibodies to concurrently label a number of unwanted cell types, for example, for the untouched isolation of target cells.

Separation strategies

Positive selection

Positive selection
The fastest way to pure target cells
Positive selection means that a particular target cell type is magnetically labeled. During separation, the magnetically labeled cells are retained within the column.
Unlabeled cells flow through. After a washing step, the column is removed from the magnetic field of the separator, and the target cells are eluted from the column. Positive selection can be performed by direct or indirect magnetic labeling. Specific MACS MicroBeads are available for the positive selection of numerous cell types.

Depletion

Depletion
Removal of an unwanted cell type
To remove a certain cell type from a mixture of cells, the unwanted cell type is magnetically labeled. During separation, the unlabeled target cells are collected in the flow-through fraction. The unwanted cell type is retained within the column.
Optionally, the retained cells can be eluted after removal of the column from the separator (not shown).

Untouched isolation

Untouched isolation
Isolation of cells in an unlabeled form
To isolate a particular target cell type in an unlabeled, i.e., untouched form, non-target cells are magnetically labeled and depleted. During separation, the unlabeled target cell type is collected in the flow-through fraction. The mixture of magnetically labeled non-target cells is retained within the column.
Optionally, the magnetically labeled cells can be eluted after removal of the column from the magnetic field of the separator (not shown).
MACS Cell Isolation Kits for untouched isolation contain a cocktail of titrated antibodies and MACS MicroBeads for indirect magnetic labeling. They are the preferred choice if binding of antibodies to the target cells is not desired.

Sequential sorting

Isolation of a cell subset by using more than one marker
A combination of two subsequent separations is applied to isolate cell subsets that can be distinguished from other cell types through their expression of two different markers. This includes cell types for which a specific marker has not been defined.

Depletion followed by positive selection:
This separation strategy is useful, if undesired cells and target cells have one marker in common. In this case, the target cells cannot be isolated in a single positive selection step using this marker.
Therefore, the undesired cells expressing this marker are magnetically labeled via antigens distinct from the common marker, and depleted. The cells appearing in the flow-through fraction during this separation are subsequently labeled with MACS MicroBeads that bind to the common marker. Target cells are then isolated by positive selection.
Sophisticated MACS Cell Isolation Kits based on this strategy are available for the fast and convenient isolation of specific cell subsets.

Two subsequent positive selections:
Multiparameter sorting with MACS MultiSort MicroBeads is based on two sequential positive selections according to two different markers.

MACS MultiSort MicroBeads specific for the first marker allow the first positive selection. After the separation, the cells are incubated with the MultiSort Release Reagent, which enzymatically removes the MultiSort MicroBeads from the cells. In the next step, the target cells are magnetically labeled with MACS MicroBeads directed against the second marker and again subjected to positive selection.

Find products in 细胞分选试剂
429 products available


View "10" | "50" | "100" products/page
Showing 1-10 of 429
Product Order no. Price

Adipose Tissue Progenitor Isolation Kit, mouse

Capacity: for up to 1×108 total cells
 
130-106-639 price on request here

Annexin V MicroBead Kit

Capacity: for 1×109 total cells
 
130-090-201 price on request here

Anti-A2B5 MicroBead Kit, human and mouse

Capacity: for 1×109 total cells
 
130-097-864 price on request here

Anti-A2B5 MicroBeads, human, mouse, rat

Capacity: for 5×108 total cells
 
130-093-388 price on request here

Anti-A2B5 MicroBeads, human, mouse, rat – small size

Capacity: for 5×107 total cells
 
130-093-392 price on request here

Anti-ABCG2 (CD338) MicroBeads, human

Capacity: for up to 1×109 total cells
 
130-107-680 price on request here

Anti-ACSA-2 MicroBead Kit, mouse

Capacity: for 1×109 total cells
 
130-097-678 price on request here

Anti-ACSA-2 MicroBead Kit, mouse – small size

Capacity: for 5×107 total cells
 
130-097-679 price on request here

Anti-AN2 MicroBeads, human and mouse

Capacity: for 1×109 total cells
 
130-097-170 price on request here

Anti-AN2 MicroBeads, human and mouse – small size

Capacity: for 1×108 total cells
 
130-097-171 price on request here
View "10" | "50" | "100" products/page
Showing 1-10 of 429


You have inquired prices for more than 1000 products. Therefore, the PDF generation can take a while. You may wait until the PDF download is available, or you can request an e-mail which provides you with a download link as soon as the PDF is ready.
Please enter a valid email address
E-Mail:
 
Thank you for your request. We will send you a download link as soon as the pdf is ready.
 
The requested price list is being generated now. You may close this note and keep browsing through our shop. A new window will open once the document is ready for download.
 

Customer login

Go

Forgot password?
Register for account

0 Shopping cart

Contact

Miltenyi Biotec Technology & Trading (Shanghai) Co., Ltd.
Phone: +86 21 62351005
Fax:+86 21 62350953
macs@miltenyibiotec.com.cn
Contact overview

Product finder

Antibody panel builder

Toolbox

Loading...

Learn MACS® Technology

Learn MACS® Technology

MACS Academy

Join a live webinar

Change country

Please select your country to continue