产品与服务 - MACS流式细胞技术 - Flow Sorter - MACSQuant® TytoCard

MACSQuant® TytoCard

  • Fully closed and disposable cartridge system
  • High speed sorting: 30.000 valve actuations/s
  • Low pressure (< 3 psi) for highest viability

Overview

The MACSQuant® TytoCard houses the actual heart of the sorting system: the microchip. The patented microchip technology enables high-speed, fluorescence-based cell sorting with the world’s fastest mechanical sort valve (30,000 valve actuations per second) in a fully closed environment, providing full safety to the operator. In addition, the TytoCard is a single-use disposable that diminishes any risk of sample cross-contamination. Both positive and negative sorted fractions remain in the TytoCard after the actual sort, allowing full retrieval of all cells within the sample.

Details

The MACSQuant® TytoCard design
The TytoCard has three compartments that can each be accessed through individual, aseptic Luer Locks from above: the input chamber, the positive-sort chamber, and the negative-sort chamber.
Cells suspended in the specially formulated MACSQuant® Tyto™ Running Buffer are loaded into the input chamber and are kept in suspension through a rotating propeller. After loading the TytoCard into the instrument, filtered air is applied to the input chamber through the pressure inlet. Driven by the air pressure, fluorescently labeled cells flow from the input chamber, through a microchannel, into the microchip where they are interrogated by three lasers. Fluorescent and scatter light signatures are used to determine whether a cell is positive or negative. To redirect a positive cell into the appropriate chamber, the solenoid located on the instrument applies a magnetic field to the chip, which triggers the valve to open up the channel to the positive sort chamber. After the positive cell has passed through, a silicon spring returns the valve into its original position. Unlike during conventional droplet sorting procedures, the cells do not experience high pressure, they are not decompressed, and no charge is applied, resulting in great viability and functionality of cells.
A detailed figure depicting the sorting mechanism of the MACSQuant TytoCard can be found in the Gallery Tab.

Gallery

Figure 1

Figure 1
The truly gentle sorting mechanism of the MACSQuant® Tyto™. Coming from the input chamber, cells enter the microchip through a microchannel where they get detected by the three lasers. Before entering the microchannel, potential cell aggregates are held back by a filter system guaranteeing a smooth sorting process. When a target cell (green) is identified, a magnetic pulse coming from the solenoid opens the microvalve, which then redirects the target cell into the positive collection chamber. In the default state, the valve is closed allowing non-selected cells (blue and red) to flow through into the negative collection chamber.
The truly gentle sorting mechanism of the MACSQuant® Tyto™. Coming from the input chamber, cells enter the microchip through a microchannel where they get detected by the three lasers. Before entering the microchannel, potential cell aggregates are held back by a filter system guaranteeing a smooth sorting process. When a target cell (green) is identified, a magnetic pulse coming from the solenoid opens the microvalve, which then redirects the target cell into the positive collection chamber. In the default state, the valve is closed allowing non-selected cells (blue and red) to flow through into the negative collection chamber.

Figure 2

Figure 2
MACSQuant® TytoCard, side view
MACSQuant® TytoCard, side view

Figure 3

The MACSQuant® TytoCard, bottom view

Figure 4

Figure 4
MACSQuant® TytoCard, bottom view showing the input chamber with propeller (left), the microchannel coming from the input chamber and leading to the chip, the positive sort chamber (middle), the microchannel leading from the chip to the negative sort chamber and the negative sort chamber (right).
MACSQuant® TytoCard, bottom view showing the input chamber with propeller (left), the microchannel coming from the input chamber and leading to the chip, the positive sort chamber (middle), the microchannel leading from the chip to the negative sort chamber and the negative sort chamber (right).

Miltenyi TV

Video 1

Immediate delivery
MACSQuant Tyto disposable cartridge

MACSQuant Tyto disposable cartridge

Watch the explanation of the exceptional features of the MACSQuant Tyto cartridge.
Product Order no. Price

MACSQuant TytoCard 24

Content: 24 pieces
 
Data sheet
130-106-088 price on request here

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Miltenyi Biotec Technology & Trading (Shanghai) Co., Ltd.
Phone: +86 21 62351005
Fax:+86 21 62350953
macs@miltenyibiotec.com.cn
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