Applications and protocols for flow cytometry

From straightforward to more complex flow cytometry protocols and applications, we are committed to helping you with your flow cytometry experiments. Check out the links below for techniques such as

  • Rare cell analysis
  • Cell cycle or apoptosis analysis
  • Immunophenotyping
  • Cancer stem cell and TIL characterization from tumors
  • Fluorescent protein analysis
  • Characterization of microvessicles

Flow Cytometry Protocols

Automated compensation
Instrument compensation is crucial for the optimal acquisition and display of data obtained by flow cytometry. Compensation essentially accounts for the inherent overlap in emission spectra observed ...
Automated sample processing
Many flow cytometry applications require a high sample throughput and a high level of standardization. The
MACSQuant® Analyzer meets these requirements offering the capability of processing 96 samples in one go with fully automated labeling and data acquisition ...
Express Modes
The MACSQuantify™ Software includes a number of data acquisition and analysis tools that have been developed for an optimized and automated measurement of cells ...
General instructions for MACSPlex Assay – Data acquisition and analysis
MACSPlex Assays are designed for determining concentrations of soluble analytes in a single sample. A prerequisite for the performance of MACSPlex Assays is a flow cytometer with ...
No-lyse whole blood analysis
For routine flow cytometric monitoring of cell populations within whole blood, there are several critical points to be considered for processing the sample material. The procedure should be ...
Protocol for cell viability
Flow cytometry is a rapid and reliable method to quantify viable cells in a cell suspension. Determination of cell viability is critical when evaluating....

Immunology

7-color immunophenotyping
Flow cytometric immunophenotyping and enumeration of a comprehensive range of immune cell subsets can be a tedious challenge. Establishing an appropriate assay usually requires...
A simple no-wash method for the flow cytometric enumeration of leukocyte subsets in whole blood
Many diseases can affect numbers of white blood cells (leukocytes) in peripheral blood and several assays are available to determine total leukocyte numbers. However, it is far less easy to reliably ...
 
Analysis of hematopoietic cell differentiation using fluorescent protein reporters and multicolor flow cytometry
Hematopoietic stem and progenitor cells have the ability to differentiate into different types of mature blood cells. The process by which stem cells choose between different ...
 
Analysis of IL-17-secreting cells
Interleukin 17 (IL-17, IL-17A, CTLA8), a member of the IL-17 family (IL-17A–F), is a disulfide-linked homodimeric glycoprotein. Human IL-17 consists of ...
High-sensitivity detection and analysis of rare cells by integrated magnetic enrichment and flow cytometry using the MACSQuant Analyzer
The detection of rare cell types in standard flow cytometric assays is restricted by the total number of cells acquired during a single measurement. Pre-enrichment of a rare target population dramatically ...
Modulation of allergic airway inflammation by the histamine H4 receptor: analysis by flow cytometry
The incidence of allergic airway diseases, such as asthma, is increasing throughout the world. Although histamine (HA) is found at high concentrations in ...
Sensitive analysis and isolation of ROR1+ B cells for research on chronic lymphocytic leukemia
Flow cytometric analysis of lymphoid cells from chronic lymphocytic leukemia (CLL) samples requires a complex panel of multiple markers. Most of these markers are expressed on ...
Characterization of antigen-specific naive and memory T cell subsets
Detection, enrichment, and direct phenotypic and functional characterization of antigen-specific naive and memory T cell subsets...
Magnetic enrichment of antigen-specific CD4+ T cells using the MACSQuant® Analyzer 10
Follicular T helper cells (TFH) play a crucial role in supporting germinal center induction and responses by ...

Cancer research

Identification and enumeration of melanoma-specific tumor-infiltrating lymphocytes and cancer stem cells
Many of the different cell types found in melanomas are of paramount interest for research into tumor biology and ultimately the development of potential treatment options. Adoptive cell therapy with tumor-infiltrating lymphocytes (TIL) ...
High-sensitivity detection and analysis of rare cells by integrated magnetic enrichment and flow cytometry using the MACSQuant Analyzer
Detection of rare cells by standard flow cytometry is restricted by the total number of cells acquired in a single measurement. Pre-enrichment of these cells greatly increases sensitivity ..
Characterization of Exosomes
Exosomes are small membrane vesicles with endocytic origin that are released by many cell types. Depending on the originating cell, exosomes are loaded with a specific set of proteins, lipids, and nucleic acids (including small RNAs) and are released constitutively or after stimulation. ..

Neuroscience

Gating strategy for quantification of myelin debris
General instructions for data acquisition and analysis of myelin debris.

Stem Cell research

Multicolor flow cytometry analysis of human pluripotent stem cell cultures
Thorough characterization of pluripotency is mandatory during the establishment of newly derived human embryonic or induced pluripotent stem cell (hiPSC) lines. ...
Complete workflow for purification, cultivation, and analysis Neonatal cardiomyocytes
Neonatal cardiomyocytes (CMs) have long been used to study biochemical, physiological, pharmacological, or morphological characteristics. Cultured CMs ...
Analysis of hematopoietic cell differentiation using fluorescent protein reporters and multicolor flow cytometry
Hematopoietic stem and progenitor cells have the ability to differentiate into different types of mature blood cells. The process by which stem cells choose between different ...
Enumeration of CD34/CD133 positive cells
The CD34 antigen is a single-chain transmembrane glycoprotein, expressed on human hematopoietic stem
and progenitor cells constituting a small subpopulation of bone marrow cells and peripheral blood cells...

Vascular Biology

Enumeration of Circulating Endothelial Cells
Circulating endothelial cells (cEC) are found in human peripheral blood and have been demonstrated to increase in number when vascular damage occurs in conjunction with ...
Enumeration of Endothelial Progenitor Cells
Experimental and clinical observations have suggested that progenitor cells in the bone marrow are mobilized and involved in various tissue regeneration processes. These stem or progenitor cells are activated and migrate ...

Cell and Molecular Biology

Analysis of fluorescent proteins
The use of flow cytometric techniques for the analysis of cell populations enables scientists to evaluate cells based on both intrinsic and extrinsic properties. Using fluorescent markers such as dyes, antibodies, or fluorescent reporters multiple characteristics of thousands of cells can be ...
Cell cycle analysis
Cell proliferation is characterized by four distinct phases: G1 phase (Gap1), S phase (DNA synthesis), G2 phase (Gap2) and M phase (mitosis). Non-proliferative or “resting cells” are referred to as ...
Determination of ploidy levels in plant cells
Misdivisions during meiosis or mitosis can lead to a multiplication of the entire set of chromosomes.
This phenomenon is called pluriploidy and occurs in many plants, in particular ...

Other applications

Characterization of microvesicles
Microvesicles (MVs) are small (0.1–1 μm) membrane vesicles shed from activated cells, delimited by a lipid bilayer and containing a wide range of ...
Comparison between antibodies from different providers: determination of median fluorescent intensities and stain indices
Flow cytometry is a powerful technique to identify, enumerate, and characterize cells by immunophenotyping. However, data quality and reliability of the results critically depend on ...
Determination of intracellular antibody production, cell density, and viability of recombinant CHO-DG44 cells using the MACSQuant Analyzer
Expression of recombinant antibodies in CHO cells is a state-of-the-art procedure in research and industry. Generation of cell lines producing high amounts of antibodies is one of the ...
Identification of different Plasmodium falciparum life cycle stages in human red blood cells using the MACSQuant Analyzer
Plasmodium falciparum is the most deadly of the four Plasmodium species that can cause malaria and is responsible for almost a million deaths every year. P. falciparum is an ...
MACSQuant® Flow Cytometers FRET Express Mode
Fluorescence resonance energy transfer (FRET) is a mechanism describing an energy transfer between two fluorescent molecules. The efficiency of this energy transfer is inversely proportional to the distance between donor and acceptor, making FRET extremely sensitive to small ...

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