Videos and tutorials on flow cytometry

We’ve assembled here a collection of our most popular tutorial videos on flow cytometry. Learn what to consider during acquisition, such as compensation or gating. See how to set up your experiment and automate your workflow. Also watch tutorials on data analysis with the MACSQuantify software.

Compensation of spectral overlap in flow cytometry

MACS Academy: Compensation of spectral overlap in flow cytometry
Join Dr. Frank Hardung and his webinar on the compensation of spectral overlap in flow cytometry. Learn about the details and how to setup a compensation workflow on a digital instrument such as the MACSQuant Analyzer 10, including the use of the MACSQuantifyTM Software for automated compensation.

Rare cell analysis in flow cytometry

MACS Academy: Rare cell analysis in flow cytometry
Join Frank Hardung and his webinar on the analysis of rare cell analysis in flow cytometry, its general approaches and proper controls.

Successful cell analysis – optimize the basic steps

Join Dr. Frank Hardung and his webinar on easy preparation of cell samples, on magnetic labeling and separation of cells, and on quality control of cell separations. Optimizing these basic steps is a prerequisite to successful cell analysis.

Sample preparation for flow cytometry

Join Dr. Frank Hardung and his webinar on sample preparation for flow cytometry – on tissue preparation, dissociation, and sample clearance.

Save time and effort in your flow cytometry experiments

MACS® Academy: Save time and effort in your flow cytometry experiments
Join Dr. Susanne Steinhaus and her webinar on MACS® Solutions for flow cytometry, including detailed information on bright dyes for multicolor analysis on the MACSQuant Instruments.

Detection and isolation of viable IL-17-secreting T cells

In this video published at the Journal of Visualized Experiments, Anna Foerster, Mario Assenmacher, Michaela Niemoeller, Elly Rankin, Mariette Mohaupt, and Anne Richter demonstrate the use of the MACS Cytokine Secretion Assay for detection and isolation of viable mouse T cells that secrete IL-17.

Immunophenotyping by flow cytometry

MACS® Academy: Immunophenotyping by flow cytometry
Join Dr. Christiane Siewert and her webinar on the advantages and challenges of multi-parameter flow cytometry. Learn how to set up the experiment and how to perform multi-color analysis of leukocyte subsets.

Quantitative analysis of ES and iPS cell cultures

MACS Academy: Quantitative analysis of ES and iPS cell cultures
Join Dr. Sebastian Knöbel and his webinar on the details of flow analysis for pluripotent stem cells.

Reliable analysis of tumor subpopulations

Join Dr. Olaf Hardt and his seminar on the dissociation and analysis of tumor tissue, using Miltenyi BIotec's instruments and reagents for sample preparation and flow cytometry.

96-well flow cytometry analysis on the MACSQuant Analyzer

96-well flow cytometry analysis on the MACSQuant Analyzer

The MACSQuant Analyzer facilitates analysis of up to 96 samples in a single run; ideally suited for large-scale assays and high-throughput screening.

Absolute cell counting on the MACSQuant Analyzer

Absolute cell counting on the MACSQuant Analyzer
It is often necessary to know the exact number of cells in a sample for downstream applications. For ease of use, the MACSQuant Analyzer quickly performs absolute cell counting without the need for counting beads or cell staining.

Autocalibration on the MACSQuant Analyzer

Autocalibration on the MACSQuant Analyzer
The automated calibration feature of the MACSQuant Analyzer saves time, is simple to operate, and ensures optimal and consistent instrument performance.

Auto-clean and shutdown on the MACSQuant Analyzer

Auto-clean and shutdown on the MACSQuant Analyzer
With automatic clean and shutdown programs available on the MACSQuant Analyzer, there is no need to stand-by until your experiment is finished.

Autocompensation on the MACSQuant Analyzer

Autocompensation on the MACSQuant Analyzer
It is widely acknowledged that spectral overlap is problematic for flow cytometric analysis using certain fluorochromes. With the MACSQuant Analyzer, 7-color compensation is fully automated.

Autolabeling on the MACSQuant Analyzer

Autolabeling on the MACSQuant Analyzer
The autolabeling feature of the MACSQuant Analyzer eliminates the time-consuming step of manual cell staining, streamlines your flow cytometry analysis, and provides higher accuracy.

Express mode on the MACSQuant Analyzer

Express mode on the MACSQuant Analyzer
After performing cell separations it is vital to determine the purity and yield of target cell populations by flow cytometry. With the MACSQuant Analyzer and MACS Control Cocktails, it is simple to qualify the separation efficiency.

Remote support for the MACSQuant Analyzer

Remote support for the MACSQuant Analyzer
The intuitive MACSQuantify Software of the MACSQuant Analyzer also offers the option to contact an application and technical support specialist in real-time.

MACSQuantify™ software – New features in version 2.5

Join Rebecca McHugh and her webinar on the latest version of the MACSQuantify software for cell analysis on the MACSQuant Analyzer.

Importing files

Learn how to import and manage your files.

Creating and Managing Gates

Learn about numerous gating options.

The Plot Side Drawer

Learn how to directly interact with your plots.

The Plot Side Drawer - Gate List Tab

Learn about advanced gating options, such as back gating and coloring of populations.

File Navigator – Batch and Jump

Learn how to efficiently navigate through your files.

Groups

Quickly analyze multiple files with the group feature

Overlays

Create and manage your overlays.

Statistics – Generating, Viewing and Exporting

Learn how to set up and export your statistic tables.

GraphLogic™

Learn how to create graphs and perform statistical tests.

PlateLogic™ – Heatmaps, tags, and filtering

Learn how to screen large numbers of samples with the plate analysis functions.

DocLogic™

Quickly create complete analysis reports.

Cell Cycle Analysis

Learn more about the automated cell cycle analysis functions.

Saving Your Analysis

Learn more about the available back up options.

Titration Analysis

Learn how to quickly analyze your titration experiments with the automated analysis feature.

Compensation

Learn how to compensate your data.

Auto-compensation

Learn how to compensate your files automatically.

Boolean Gates

Learn how to set and handle boolean gates.

MACSQuant – Color your research

MACSQuant – Color your research.
Add color to your research with the Miltenyi Biotec flow cytometry solution, featuring the MACSQuant family of flow cytometers and Vio Dye antibody fluorochromes.

MACSQuant Analyzer: Excite and inspire!

MACSQuant Analyzer: Excite and inspire!
The MACSQuant Analyzer is a novel and unique research tool with ten optical channels, powerful and intuitive software, absolute cell counting, sensitive rare cell analysis using magnetic separation, and multisample processing of up to 96 samples.

MACS Cell Analysis – At the heart of your experiments

MACS® Cell Analysis – At the heart of your experiments
For over 25 years, we’ve strived to bring continuous innovation to the world of life science research. With over 20,000 publications to date, our technologies are used by scientists around the world at each step and every breakthrough.

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